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Streptomyces ghanaensis VITHM1 1

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Generation of CRISPR/Cas9-mediated lactoferrin-targeted mice by pronuclear injection of plasmid pX330

Mengxu GE,Fei LIU,Fei CHANG,Zhaolin SUN,Jing FEI,Ying GUO,Yunping DAI,Zhengquan YU,Yaofeng ZHAO,Ning LI,Qingyong MENG

Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 3,   Pages 242-248 doi: 10.15302/J-FASE-2015059

Abstract: with lactoferrin promoter mutations were obtained with an efficiency of 24% (7/29) by injecting the plasmidPlasmid integration and off-targeting of pX330 were not detected.These results confirmed that pronuclear injection of a circular plasmid is a feasible and efficient method

Keywords： lactoferrin     promoter     CRISPR/Cas9     plasmid pX330    

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Lentivector-mediated RNAi efficiently downregulates expression of murine cdk4 gene

Feng JIANG PhD , Xuezhen WANG PhD , Zheng XUE MD , Suming ZHANG PhD , Siyu FANG BM , Min ZHANG MD, PhD ,

Frontiers of Medicine 2009, Volume 3, Issue 3,   Pages 287-291 doi: 10.1007/s11684-009-0050-5

Abstract: Lentivector-mediated RNAi could efficiently down-regulate the expression of the murine cdk4 gene , which

Keywords： cyclin-dependent kinase 4     RNA interference     plasmid     lentiviral vector    

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Construction of Hsp90β gene specific silencing plasmid and its transfection efficiency

JI Yewei, NIE Bin, LI Ping, ZHOU Yuanguo, XU Xiaoyu

Frontiers of Medicine 2007, Volume 1, Issue 3,   Pages 253-257 doi: 10.1007/s11684-007-0048-9

Abstract: The purpose of this work was to construct the plasmid that could direct the synthesis of siRNA-like transcriptsof the target gene were chemically synthesized and annealed and were then ligated with pSUPER EGFP1 plasmidThen the plasmids were transfected into the cells at different ratios of plasmid to Lipofectamine.After optimizing the ratio of plasmid to Lipofectamine, we achieved high transfection efficiency in HeK293

Keywords： HindIII     transfection efficiency     detection     enhanced     different    

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Degradation of extracellular genomic, plasmid DNA and specific antibiotic resistance genes by chlorination

Menglu Zhang, Sheng Chen, Xin Yu, Peter Vikesland, Amy Pruden

Frontiers of Environmental Science & Engineering 2019, Volume 13, Issue 3, doi: 10.1007/s11783-019-1124-5

Abstract:

Plasmid DNA/ARGs were less readily broken down

Keywords： Antibiotic resistance     Antibiotic resistance genes (ARGs)     Extracellular DNA/ARGs     Chlorination    

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Influences and mechanisms of nanofullerene on the horizontal transfer of plasmid-encoded antibiotic resistance

Qingkun Ji, Caihong Zhang, Dan Li

Frontiers of Environmental Science & Engineering 2020, Volume 14, Issue 6, doi: 10.1007/s11783-020-1287-0

Abstract: Abstract • Sub-inhibitory levels of nC60 promote conjugative transfer of ARGs. • nC60 can induce ROS generation, oxidative stress and SOS response. • nC60 can increase cell membrane permeability and alter gene expression. • Results provide evidence of nC60 promoting antibiotic resistance dissemination. The spread and development of antibiotic resistance globally have led to severe public health problems. It has been shown that some non-antibiotic substances can also promote the diffusion and spread of antibiotic resistance genes (ARGs). Nanofullerene (nC60) is a type of nanomaterial widely used around the world, and some studies have discovered both the biological toxicity and environmental toxicity of nC60. In this study, cellular and molecular biology techniques were employed to investigate the influences of nC60 at sub-minimum inhibitory concentrations (sub-MICs) on the conjugation of ARGs between the E. coli strains. Compared with the control group, nC60 significantly increased the conjugation rates of ARGs by 1.32‒10.82 folds within the concentration range of 7.03‒1800 mg/L. This study further explored the mechanism of this phenomenon, finding that sub-MICs of nC60 could induce the production of reactive oxygen species (ROS), trigger SOS-response and oxidative stress, affect the expression of outer membrane proteins (OMPs) genes, increase membrane permeability, and thus promote the occurrence of conjugation. This research enriches our understanding of the environmental toxicity of nC60, raises our risk awareness toward nC60, and may promote the more rational employment of nC60 materials.

Keywords： Nanofullerene     Sub-minimum inhibitory concentrations     Antibiotic resistance genes     Conjugation     Molecular biological techniques    

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Ultrasound-mediated targeted microbubbles: a new vehicle for cancer therapy

Junxiao YE, Huining HE, Junbo GONG, Weibing DONG, Yongzhuo HUANG, Jianxin WANG, Guanyi CHEN, Victor C YANG

Frontiers of Chemical Science and Engineering 2013, Volume 7, Issue 1,   Pages 20-28 doi: 10.1007/s11705-013-1311-z

Abstract: ultrasound microbubble contrast agent can carry gene drugs and so on to the target tissue, as well as mediated

Keywords： ultrasound-mediated     targeted microbubbles     cancer    

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Regulation of exogenous bFGF gene mediated by recombinant adeno-associated virus

Ke SONG, Nianjing RAO, Meiling CHEN, Yingguang CAO

Frontiers of Medicine 2009, Volume 3, Issue 2,   Pages 158-163 doi: 10.1007/s11684-009-0042-5

Abstract: The regulatory effect of basic fibroblast growth factor (bFGF) mediated by recombinant adeno-associatedRecombinant plasmid pAAV-S3-bFGF, and pSVneo were co-transfected into BHK-21 cells, then the recombinant

Keywords： tetracycline regulatory system     adeno-associated virus     basic fibroblast growth factor     gene regulation    

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Sulfur mediated heavy metal biogeochemical cycles in coastal wetlands: From sediments, rhizosphere to

Frontiers of Environmental Science & Engineering 2022, Volume 16, Issue 8, doi: 10.1007/s11783-022-1523-x

Abstract:

• In sediments, the transformation of sulfides may lead to the release of heavy metals.

Keywords： Coastal wetland     Heavy metal     Sulfur     Biogeochemical cycle    

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Microbial mediated arsenic biotransformation in wetlands

Si-Yu Zhang, Paul N. Williams, Jinming Luo, Yong-Guan Zhu

Frontiers of Environmental Science & Engineering 2017, Volume 11, Issue 1, doi: 10.1007/s11783-017-0893-y

Abstract: Among these fine-scale events, microbial mediated As biotransformations contribute most to the elementdocument some of these key advances, with focuses on the implications that wetlands and their microbial mediatedtransformation pathways have on the global As cycle, the chemistries of microbial mediated As oxidation

Keywords： Arsenic     Wetland     Microbes     Switch    

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on porphyrinic triazine-based frameworks with excellent biocompatibility for conversion of CO in H-mediated

Frontiers of Chemical Science and Engineering 2022, Volume 16, Issue 12,   Pages 1761-1771 doi: 10.1007/s11705-022-2195-6

Abstract: Microbial electrosynthesis is a promising alternative to directly convert CO2 into long-chain compounds by coupling inorganic electrocatalysis with biosynthetic systems. However, problems arose that the conventional electrocatalysts for hydrogen evolution may produce extensive by-products of reactive oxygen species and cause severe metal leaching, both of which induce strong toxicity toward microorganisms. Moreover, poor stability of electrocatalysts cannot be qualified for long-term operation. These problems may result in poor biocompatibility between electrocatalysts and microorganisms. To solve the bottleneck problem, Co anchored on porphyrinic triazine-based frameworks was synthesized as the electrocatalyst for hydrogen evolution and further coupled with Cupriavidus necator H16. It showed high selectivity for a four-electron pathway of oxygen reduction reaction and low production of reactive oxygen species, owing to the synergistic effect of Co–Nx modulating the charge distribution and adsorption energy of intermediates. Additionally, low metal leaching and excellent stability were observed, which may be attributed to low content of Co and the stabilizing effect of metalloporphyrins. Hence, the electrocatalyst exhibited excellent biocompatibility. Finally, the microbial electrosynthesis system equipped with the electrocatalyst successfully converted CO2 to poly-β-hydroxybutyrate. This work drew up a novel strategy for enhancing the biocompatibility of electrocatalysts in microbial electrosynthesis system.

Keywords： microbial electrosynthesis     hydrogen evolution reaction     metalloporphyrins     biocompatibility     CO2 conversion    

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Discovery of novel ursolic acid derivatives as effective antimicrobial agents through a ROS-mediated

Frontiers of Chemical Science and Engineering 2023, Volume 17, Issue 12,   Pages 2101-2113 doi: 10.1007/s11705-023-2361-5

Abstract: In response to the reduction of food production and economic losses caused by plant bacterial diseases, it is necessary to develop new, efficient, and green pesticides. Natural products are rich and sustainable source for the development of new pesticides due to their low toxicity, easy degradation, and eco-friendliness. In this study, we prepared three series of ursolic acid derivatives and assessed their antibacterial ability. Most target compounds exhibited outstanding antibacterial activities. Among them, the relative optimal EC50 values of Xanthomonas oryzae pv. oryzae and Xanthomonas axonopodis pv. citri were 2.23 (A17) and 1.39 (A16) μg·mL–1, respectively. The antimicrobial mechanism showed that compound A17 induced an excessive accumulation and production of reactive oxygen species in bacteria and damaged the cell membrane integrity to kill bacteria. More interestingly, the addition of low concentrations of exogenous hydrogen peroxide enhanced the antibacterial efficacy of compound A17 against Xanthomonas oryzae pv. oryzae. These entertaining results suggested that compound A17 induced an apparent apoptotic behavior in the tested bacteria. Overall, we developed the promising antimicrobial agents that destroyed the redox system of phytopathogenic bacteria, further demonstrating the unprecedented potential of ursolic acid for agricultural applications.

Keywords： ursolic acid     antibacterial activities     reactive oxygen species     apoptosis    

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Machine learning enabled prediction and process optimization of VFA production from riboflavin-mediated

Frontiers of Environmental Science & Engineering 2023, Volume 17, Issue 11, doi: 10.1007/s11783-023-1735-8

Abstract:

● Data-driven approach was used to simulate VFA production from WAS fermentation.

Keywords： Machine learning     Volatile fatty acids     Riboflavin     Waste activated sludge     eXtreme Gradient Boosting    

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Berberine alleviates myocardial diastolic dysfunction by modulating Drp1-mediated mitochondrial fission

Frontiers of Medicine doi: 10.1007/s11684-023-0983-0

Abstract: Berberine alleviates myocardial diastolic dysfunction by modulating Drp1-mediated mitochondrial fission

Keywords： mitochondrial fission Ca     Berberine alleviates myocardial     murine model HFpEF    

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Sodium butyrate activates HMGCS2 to promote ketone body production through SIRT5-mediated desuccinylation

Frontiers of Medicine 2023, Volume 17, Issue 2,   Pages 339-351 doi: 10.1007/s11684-022-0943-0

Abstract: The data suggests that SB activated HMGCS2 through SIRT5-mediated desuccinylation for ketone body production

Keywords： sodium butyrate     succinylation     HMGCS2     ketogenesis     SIRT5    

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VITHM1 mediated green synthesis of silver nanoparticles: Mechanism and biological applications

Mani Abirami, Krishnan Kannabiran

Frontiers of Chemical Science and Engineering 2016, Volume 10, Issue 4,   Pages 542-551 doi: 10.1007/s11705-016-1599-6

Abstract: secondary metabolites in the cell free supernatant of this bacterium when incubated with 1 mmol/L AgNO , mediated

Keywords： Streptomyces ghanaensis VITHM1     nanoparticles     3D structure     antibacterial activity    

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Title Author Date Type Operation

Generation of CRISPR/Cas9-mediated lactoferrin-targeted mice by pronuclear injection of plasmid pX330

Mengxu GE,Fei LIU,Fei CHANG,Zhaolin SUN,Jing FEI,Ying GUO,Yunping DAI,Zhengquan YU,Yaofeng ZHAO,Ning LI,Qingyong MENG

Journal Article

Lentivector-mediated RNAi efficiently downregulates expression of murine cdk4 gene

Feng JIANG PhD , Xuezhen WANG PhD , Zheng XUE MD , Suming ZHANG PhD , Siyu FANG BM , Min ZHANG MD, PhD ,

Journal Article

Construction of Hsp90β gene specific silencing plasmid and its transfection efficiency

JI Yewei, NIE Bin, LI Ping, ZHOU Yuanguo, XU Xiaoyu

Journal Article

Degradation of extracellular genomic, plasmid DNA and specific antibiotic resistance genes by chlorination

Menglu Zhang, Sheng Chen, Xin Yu, Peter Vikesland, Amy Pruden

Journal Article

Influences and mechanisms of nanofullerene on the horizontal transfer of plasmid-encoded antibiotic resistance

Qingkun Ji, Caihong Zhang, Dan Li

Journal Article

Ultrasound-mediated targeted microbubbles: a new vehicle for cancer therapy

Junxiao YE, Huining HE, Junbo GONG, Weibing DONG, Yongzhuo HUANG, Jianxin WANG, Guanyi CHEN, Victor C YANG

Journal Article

Regulation of exogenous bFGF gene mediated by recombinant adeno-associated virus

Ke SONG, Nianjing RAO, Meiling CHEN, Yingguang CAO

Journal Article

Sulfur mediated heavy metal biogeochemical cycles in coastal wetlands: From sediments, rhizosphere to

Journal Article

Microbial mediated arsenic biotransformation in wetlands

Si-Yu Zhang, Paul N. Williams, Jinming Luo, Yong-Guan Zhu

Journal Article

on porphyrinic triazine-based frameworks with excellent biocompatibility for conversion of CO in H-mediated

Journal Article

Discovery of novel ursolic acid derivatives as effective antimicrobial agents through a ROS-mediated

Journal Article

Machine learning enabled prediction and process optimization of VFA production from riboflavin-mediated

Journal Article

Berberine alleviates myocardial diastolic dysfunction by modulating Drp1-mediated mitochondrial fission

Journal Article

Sodium butyrate activates HMGCS2 to promote ketone body production through SIRT5-mediated desuccinylation

Journal Article

VITHM1 mediated green synthesis of silver nanoparticles: Mechanism and biological applications

Mani Abirami, Krishnan Kannabiran

Journal Article